Extended Data Fig. 8: Macro- and microscopic analyses of pig hearts after chronic myocardial ischemia and HVP-injection.
a, Image of freshly explanted pig heart 84 days after in-vivo HVP-injection indicating injection sites (A, B), showing no macroscopic signs of teratoma. b, Negative immunostaining for OCT4 confirms absence of undifferentiated pluripotent stem cells in the human graft after 12 weeks. Scale bar 50 µm. c, Representative immunohistochemistry images of scar (left) and border area (right) after HVP-injection with marked human nuclei (HuNu) and cTNT. Scale bars 250 µm (left) and 1 mm (right). d, Quantification of Cyclosporin A concentrations (immunosuppression) in whole blood of HVP- and vehicle-treated animals performed regularly throughout follow up period. Data are shown as mean ± SEM. n = 10 pigs in vehicle treated group and n = 7 pigs in HVP-treated group. Numerical data are provided in Source Data Extended Data Fig. 8.
