Extended Data Fig. 5: Adiponectin receptors promote HSC function by reducing IFNγ receptor signaling (related to Fig. 4).
a. Bone marrow (one femur and one tibia) and spleen cellularity in Vav1-Cre; Ifngr1fl/fl and control mice (n = 7 Vav1-Cre; Ifngrfl/fl mice and n = 6 control mice in three independent experiments). b-d. The frequencies of HSCs, MPPs, HPCs (b), CMPs, MEPs, GMPs (c), and differentiated T, B, myeloid, and erythroid cells (d) in the bone marrow of Vav1-Cre; Ifngr1fl/fl and control mice (n = 7 Vav1-Cre; Ifngrfl/fl mice and n = 6 control mice in three independent experiments). e−g. The frequencies of HSCs, MPPs, HPCs (e), CMPs, MEPs, GMPs (f), and differentiated cells (g) in the spleens of Vav1-Cre; Ifngr1fl/fl and control mice (n = 7 Vav1-Cre; Ifngrfl/fl mice and n = 6 control mice in three independent experiments). h. The percentage of HSCs that incorporated a 72 hour pulse of BrdU (n = 3 Vav1-Cre; Ifngrfl/fl mice and n = 4 control mice one experiment). i-o. The frequencies of HSCs (i), MPPs (j), HPCs (k), CMPs (l), MEPs (m), GMPs (n) and differentiated cells (o) in the bone marrow of control, Vav1-Cre; Adipor1fl/fl; Adipor2fl/fl, and Vav1-Cre; Adipor1fl/fl; Adipor2fl/fl; Ifngrfl/fl mice (n = 15 Vav1-Cre; Adipor1fl/fl; Adipor2fl/fl mice, n = 13 Vav1-Cre; Adipor1fl/fl; Adipor2fl/fl; Ifngrfl/f mice and n = 21 control mice in five independent experiments). All data represent mean ± standard deviation and each dot reflects a different mouse. Statistical significance was assessed using a matched samples two-way ANOVA followed by Sidak’s multiple comparisons adjustment (a), Mann-Whitney tests followed by Holm-Sidak’s multiple comparisons adjustments (b-g), a Student’s t-test (h), or one−way ANOVAs followed by Tukey’s multiple comparisons adjustments (i-o). All statistical tests were two-sided. Source numerical data are available in the source data files.
