Extended Data Fig. 6: Adiponectin receptors promote HSC function by reducing TNF levels (related to Fig. 5).

a. Bone marrow (one femur and one tibia) and spleen cellularity in Tnf deficient and control mice (n = 5 Tnf^-/- mice and n = 7 control mice in four independent experiments). b-d. The frequencies of HSCs, MPPs, HPCs (b), CMPs, MEPs, GMPs (c) and differentiated T, B, myeloid, and erythroid cells (d), in the bone marrow of Tnf deficient and control mice (n = 5 Tnf^-/- mice and n = 7 control mice in four independent experiments). e-g. The frequencies of HSCs, MPPs, HPCs (e), CMPs, MEPs, GMPs (f) and differentiated cells (g), in the spleens of Tnf deficient and control mice (n = 5 (e and g) or n = 4 (f) Tnf^-/- mice and n = 7 control mice in four independent experiments). h. The percentage of HSCs that incorporated a 72 hour pulse of BrdU (n = 4 Tnf^-/- mice and n = 9 control mice in two independent experiments) i-o. The frequencies of HSCs (i), MPPs (j), HPCs (k), CMPs (l), MEPs (m), GMPs (n) and differentiated cells (o) in the bone marrow of control, Vav1-Cre; Adipor1^fl/fl; Adipor2^fl/fl, and Vav1-Cre; Adipor1^fl/fl; Adipor2^fl/fl; Tnf^-/- mice (n = 12 Vav1-Cre; Adipor1^fl/fl; Adipor2^fl/fl mice, n = 11 Vav1-Cre; Adipor1^fl/fl; Adipor2^fl/fl; Tnf^-/- mice and n = 12 control mice in five independent experiments). p-q. ELISA of TNF (p) or INFg (q) in blood plasma from Vav1-Cre; Adipor1^fl/fl; Adipor2^fl/fl; Ifngr^fl/fl (p), Vav1-Cre; Adipor1^fl/fl; Adipor2^fl/fl; Tnf^-/- (q), Vav1-Cre; Adipor1^fl/fl; Adipor2^fl/fl and control mice (n = 6 Vav1-Cre; Adipor1^fl/fl; Adipor2^fl/fl mice, n = 10 Vav1-Cre; Adipor1^fl/fl; Adipor2^fl/fl; Ifngr^fl/f mice and n = 17 control mice run in one ELISA per cytokine (p) and n = 13 Vav1-Cre; Adipor1^fl/fl; Adipor2^fl/fl mice, n = 6 Vav1-Cre; Adipor1^fl/fl; Adipor2^fl/fl; Tnf^-/- mice and n = 12 control mice in one ELISA per cytokine (q)). All data represent mean ± standard deviation and each dot reflects a different mouse. Statistical significance was assessed using a matched samples two-way ANOVA followed by Sidak’s multiple comparisons adjustment (a), Student’s t-tests (b-h) followed by Holm-Sidak’s multiple comparisons adjustments (b-g), one-way ANOVAs followed by Tukey’s multiple comparisons adjustments (i-o and q), a Welch’s one-way ANOVA followed by Dunnett’s T3 multiple comparisons adjustment (o), or a Kruskal-Wallis test followed by Dunn’s multiple comparisons adjustment (o and p). All statistical tests were two-sided. Source numerical data are available in the source data files.

Source data