Extended Data Fig. 2: Flow cytometry gating strategy for the isolation of hematopoietic stem cells, progenitor cells, and differentiated cells (related to Figs. 1–6).

a-c. Representative flow cytometry gates used to identify hematopoietic stem and progenitor cells (a-b), NK cells, CD4 + T cells, and CD8 + T cells (c) in the bone marrow. The markers used to identify each of the cell populations characterized in this study are listed in Supplementary Table 1. d. Hematoxylin and Eosin stained sections from the spleens of Vav1-Cre; Adipor1^fl/fl; Adipor2^fl/fl and control mice. In Vav1-Cre; Adipor1^fl/fl; Adipor2^fl/fl mice the spleens were enlarged (Fig. 1d) and the red pulp was expanded throughout the spleen with increased numbers of myeloid, erythroid, and megakaryocytic cells. In control spleens there was limited extramedullary hematopoiesis confined to the subcapsular region. Representative images from three mice per genotype in one experiment are shown.