Extended Data Fig. 5: TCF/LEF are redistributed with SOX2 and β-catenin. | Nature Cell Biology

Extended Data Fig. 5: TCF/LEF are redistributed with SOX2 and β-catenin.

From: Sox2 levels regulate the chromatin occupancy of WNT mediators in epiblast progenitors responsible for vertebrate body formation

Extended Data Fig. 5

The majority of (a) LEF1, (b) TCF7L1, and (c) TCF7L2 differential peaks (FDR <0.05) overlap SOX2 differential peaks. FDR was determined by DESeq2 padj metric from n=3 biological replicates. The majority of (d) LEF1, (e) TCF7L1, and (f) TCF7L2 differential peaks (FDR <0.05) overlap β-catenin differential peaks. FDR was determined by DESeq2 padj metric from n=3 biological replicates. (g) LEF1, (h) TCF7L1, and (i) TCF7L2 exhibit a similar cell-type specific pattern of occupancy as β-catenin at SOX2 differential peaks classified in Fig. 3e (compare to Fig. 3e,f). Metaplots of triplicate (j) LEF1, (k) TCF7L1 and (l) TCF7L2 ChIP-seq signals at cell-type specific SOX2 bound peaks classified in Fig. 3e. Metaplots show mean ± s.e.m.

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