Fig. 1: Ions and candidate ion transporters in MP shrinkage.
From: Proton-gated anion transport governs macropinosome shrinkage
a, Scheme of experimental approach. b, Fluorescence images of MPs formed in presence of 70 kDa TMR–dextran, 5 and 15 min after M-CSF addition. Scale bars, 5 µm. c, Removal of luminal Na+ or Cl− impairs MP resolution, measured by vesicle volume (left) or TMR-dextran fluorescence (right). n = 11, N = 743 (WT); n = 7, N = 533 (low Na+, near 0 mM); n = 10, N = 869 (low Cl−, 9 mM). n is number of animals, N is number of MPs. Plot of mean ± standard error of the mean (s.e.m.) (shown as bands), averaging means from individual mice. d, Mean MP volume 15 min after M-CSF normalized to volume at 5 min as function of luminal ion concentrations. Data points, mean values from individual mice. Error bars, s.e.m. One-way ANOVA with Tukey’s multiple comparison shown with regard to NaCl. e, Ion transporter candidates. f–m, Western blot expression analysis of TMEM206 (specific bands indicated by arrows) (f), LRRC8A (g), ClC-2 (h), ClC-3 (i), ClC-4 (j), ClC-5 (k), ClC-6 (l) and ClC-7 (m) in mouse BMDMs, compared with organs highly expressing the respective proteins. α1 Na/K-ATPase (f) and actin (g–m) were used as loading control. n = 3 for each western blot. Source numerical data and unprocessed blots are available in source data.
