Extended Data Fig. 5: Disruption of Clcn2, Clcn3, Clcn4, Clcn5, Clcn7 and Lrrc8a did not affect macropinosome resolution.
From: Proton-gated anion transport governs macropinosome shrinkage
(a-e) For each genotype volume decrease (upper panels) and fluorescence intensity increase (lower panels) are not significantly different from WT (n=3 mice for Clcn2−/− (N=294) and Clcn3−/− (N=266), n=4, N=125 for WT); n=3 for Clcn4−/− (N=270) and WT (N=235); n=5, N=822 for Clcn5−/− and n=3, N=263 for WT; n=3 for Clcn7−/− (N=19) and WT (N=74); n=4, N=311 for Lrrc8a−/− and n=3, N=159 for WT. Clcn7−/− cells were selected by YFP expression. Plot of mean ± s.e.m. (shown as bands), averaging means from individual mice. (f) Western blot showing decreased expression of LRRC8A in Cx3cr1-CreERT2; Lrrc8alox/lox BMDMs after tamoxifen (Tam) induction (top panel). Actin (bottom panel) was used as loading control. 10 µg of total protein were loaded per sample. Source numerical data and unprocessed blots are available in source data.
