Fig. 2: Endogenous expression of TMEM206 and ClC-7 in mouse primary macrophages.
From: Proton-gated anion transport governs macropinosome shrinkage
a, Immunofluorescence analysis for TMEM206 in BMDMs. The channel resides on intracellular vesicles (arrow) but also at the plasma membrane (arrowhead). Tmem206−/− cells prove antibody specificity. Scale bars, 5 µm. b, Representative traces of acid-activated (pHo 4.8) anion currents in WT, but not Tmem206−/− BMDMs, obtained with whole-cell patch-clamp recordings. Voltage step protocol shown on top. N = 8 cells per genotype. c, TMEM206 co-localizes with early endosomal EEA1, but not with lysosome marker LAMP1. Pearson’s R calculated from 21 cells (EEA1) and 33 cells (LAMP1) from two independent experiments. d, ClC-7 is present in lysosomes (LAMP1) and does not co-localize with early endosome marker EEA1. Pearson’s R calculated from 27 cells (EEA1) and 23 cells (LAMP1) from two independent experiments. Mean ± s.e.m. Scale bars, 5 µm and 1 µm for enlargements. Source numerical data are available in source data.
