Fig. 3: Expression of TMEM206 and ClC-7 on BMDM MPs.
From: Proton-gated anion transport governs macropinosome shrinkage
a,b, TMEM206 on MPs 4 min (a) and 7 min (b) after M-CSF co-localize with rab5 (a and b) and rab7 (b) respectively. Pearson’s R calculated from 23 cells (Rab5, 4 min), 27 cells (Rab5, 7 min), 22 cells (Rab7, 4 min) and 21 cells (Rab7, 7 min) from at least two independent experiments. Scale bars, 10 µm and 1 µm for enlargements. c, Frames from live cell imaging of TMEM206-GFP transfected BMDMs at various timepoints after addition of M-CSF together with TMR–dextran (Supplementary Video 2). Scale bars, 5 µm and 1 µm for enlargements (bottom). d–f, ClC-7 is absent from MPs at 4 min (d) or 7 min (e) after M-CSF induction, but is on rab7-positive MPs after 15 min (f). Areas chosen for magnification indicated by white arrowheads on the left. Rab7 was absent from MPs at 4 min (d). Scale bars, 10 µm and 1 µm for enlargements. Source numerical data are available in source data.
