Extended Data Fig. 8: scRNA-seq UMAP cluster assignment and characterization.
a UMAP projection of integrated datasets as in Fig. 5a, highlighting cells from Zheng et al65. b Unassigned Seurat cluster distribution of UMAP used in Fig. 5a. c Bubble plot showing expression of key marker genes across the cell clusters as indicated. The sizes and colors of dots indicate the proportion of cells expressing the corresponding genes and their averaged scaled values of log-transformed expression, respectively. d Reanalysis of published scRNA-seq in naïve hESC from Messmer et. al53. Expression of GATA3, CDX1, HAND1, KRT19 is color coded in PCA map as defined in Messmer et. al. e Volcano plot showing differentially expressed genes (482 up, 387 down) comparing ELC population at 7d EZH2i with ELC population in untreated naïve hESC. f Violin plot showing single-cell log-transformed expression of HAND1 and CDX1 transcription factors.
