Fig. 1: Host ATF4 deletion inhibits tumour growth and extends survival.
a, Top: loxP sites flank exons 2 and 3 of the Atf4 gene. Bottom: schematic of the tamoxifen treatment schedule. Tamoxifen (200 mg per kg body weight (BW) was given for 5 consecutive days by oral gavage. b, Box and whisker plot of the RT–qPCR results of Atf4 mRNA levels in whole lung (n = 3–5 biologically independent samples per group). Unpaired two-sample t-test. c, Reversible BW loss after ATF4 excision. Two-way analysis of variance (ANOVA) analysis (until day 34). Values represent the mean ± s.e.m., unpaired t-test. NS, not significant. d, Tumour growth curves of Atf4WT/WT and Atf4Δ/Δ mice following the injection of 5 × 105 B16F10 cells. Values represent the mean ± s.e.m., two-way ANOVA analysis (until day 18). e, Kaplan–Meier survival analysis of the mice from d (log-rank (Mantel–Cox) test). f, Growth curves after injection of B16F10 cells into Atf4WT/WT and Atf4Δ/Δ mice with the tamoxifen administered after the tumours reached around 100 mm3 and continued for 5 days (dark green line on x axis). Values represent the mean ± s.e.m., two-way ANOVA analysis (until day 18). g, Tumour growth curves of Atf4WT/WT and Atf4Δ/Δ mice following injection of 5 × 105 MH6419 cells. Values represent the mean ± s.e.m., two-way ANOVA analysis (until day 24). h, Images from pancreas collected 3 weeks after injection of 5 × 104 MH6419 cells orthotopically in the tail of the pancreas of Atf4WT/WT (n = 11 biologically independent samples) and Atf4Δ/Δ (n = 7 biologically independent samples). The yellow and blue dotted lines indicate the tumour and normal areas of pancreas, respectively. i, Box and whisker plot display the percentage tumour normalized to BW. Unpaired two-sample t-test. n in figures represent biologically independent samples.
