Extended Data Fig. 5: ATF4 ablation is associated with reduced levels of CAFs markers, proliferation and collagen deposition in the TME.
a, Box and whisker plot of the % colocalization of aSMA+ with CD31+ in B16F10 tumours (n = 5–6 biologically independent samples per group). b, Representative IF images from MH6419 tumours stained for CD31 (green) and αSMA (red). Magnification, 20x. c, Box and whisker plots of the % αSMA positive area and d, of the % colocalization of aSMA+ with CD31+from b (n = 4 biologically independent samples per group). e, Representative IF images from orthotopic MH6419 pancreatic tumours stained for CD31 (green) and αSMA (red). Magnification, 10x. f, Box and whisker plot of the % colocalization of aSMA+ with CD31+ from e. g, Representative IF images from B16F10 tumours stained for CD31 (green) and PDGFRβ (red). Magnification, 10x. h, Box and whisker plot of the % PDGFRβ positive area and i, of the % colocalization of PDGFRβ+ with CD31+ from g (n = 4–6 biologically independent samples per group). j, Representative IF images from B16F10 tumours stained for αSMA (orange) and Ki67 (green). Magnification, 20x. k, Box and whisker plot of %Ki67+αSMA+ cells from j. l, Representative IF images from B16F10 tumours stained for αSMA (orange) and TUNEL (green). Magnification, 10x. m, Box and whisker plot the %TUNEL+αSMA + cells from l (n = 4 biologically independent samples per group). n, Representative IF images from B16F10 tumours stained for CD31 (green) and NG2 (red). Magnification, 20x. o, Box and whisker plot of % colocalization of NG2+ with CD31+ from n (n = 10–12 biologically independent samples per group). p. Representative images from MH6419 tumour sections stained with an antibody against collagen (green). q, Box and whisker plot of the % of positive collagen area from p (n = 4–7 biologically independent samples per group). Unpaired two-sample t-test in all box and whisker plots. Scale bars, 100 μm (b, e, g, j, l and n) and 1 mm (p).
