Extended Data Fig. 6: Fibroblast specific ATF4 deletion causes abnormal vascularization.

a, Box and whisker plot display RT-qPCR of Atf4 in DFB cells (n = 4–5 biologically independent samples per group). Unpaired two-sample t-test. b, Representative images of labelled DFBs with the Vybrant™ DiD. Magnification, 10x. c, In vivo tracking of the DFB + DiD cells injected to the flank of the mice by IVIS imaging. d, Quantification of the fluorescent signal from c. Values represent mean ± SEM, unpaired two-sample t-test. e, Genotyping of mice for Col1a1::CreER^T2 and ATF4 status. f, No BW changes post ATF4 excision in Col1a1Cre;Atf4^wt/wt and Col1a1Cre;Atf4^Δ/Δ mice (n = 6 biologically independent samples per group); n.s. = not significant. Values represent mean ± SEM, two-way ANOVA analysis. g, Representative IF images from B16F10 tumours grown in Col1a1Cre;Atf4^wt/wt and Col1a1Cre;Atf4^Δ/Δ mice stained for CD31 (green). Magnification, 10x. h, Box and whisker plot display the microvascular density from g (n = 5 biologically independent samples per group). Unpaired two-sample t-test. i, Floating bars display the RT-qPCR of Atf4, Pecam1, Col1a1 and Col1a2 in isolated tumour endothelial cells (TEC) and LFBs (n = 2–3 biologically independent samples per group). Light gray lines are used to separate the groups for each gene. Scale bars, 100 μm (b and g).

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