Extended Data Fig. 1: Tspan4 proteins are highly recruited to sites of cellular membrane damage.
From: Assembly of Tetraspanin-enriched macrodomains contains membrane damage to facilitate repair
a L929 Tspan4–mCherry and MGC803 Tspan4–GFP cells were subjected to photodamage treatment and Tspan4 distribution at the damage site was observed by confocal microscopy. Scale bar, 5 µm. b NRK cells co-expressing Tspan4–GFP and Na/K ATPase-mCherry were photodamaged, then time-lapse images were collected by confocal microscopy. Scale bar, 5 µm. c After cells were photodamaged, PI was added into the system at different time points (0, 1, 2 and 2.5 min) and the PI intensity was traced. Representative images are showed. Green, Tspan4–GFP; red, PI. Scale bar, 5 µm. d Statistical analysis of the percentage of cells with PI entry at each time point was conducted. Mean±s.e.m., n = 12 cells for the 0 min group, n = 22 for the 1 min group, n = 24 cells for the 2 min group and n = 50 cells for the 2.5 min group from 3 independent experiments. Experiments were independently performed 3 times (a-c), and representative images are shown.
