Extended Data Fig. 2: ESCRT III activity is not required for recruitment of Tspan4 to damage sites.
From: Assembly of Tetraspanin-enriched macrodomains contains membrane damage to facilitate repair
a NRK cells co-expressing Tspan4–GFP and Chmp4b–mCherry were subjected to detergent (25 µM digitonin) treatment. Z-projection images were collected by 3D-SIM. Yellow, Tspan4; cyan, Chmp4b–mCherry. Scale bar, 5 µm. b Tspan4–GFP overexpressing cells treated with DMSO (as control) or 10 µM BAPTA-AM were subjected to photodamage. Time-lapse images were collected by confocal microscopy. Scale bar, 5 µm. c Localization of Tspan4 and Chmp3 at the damage site was analysed by confocal microscopy after photodamage in cells co-expressing Tspan4–GFP and Chmp3–mCherry or Chmp3 1-179-mCherry. Scale bar, 10 µm. d Verification of the NRK Chmp4b knockout cell line by PCR. Experiments were independently performed 3 times (a-d), and representative images are shown.
