Extended Data Fig. 3: Cholesterol depletion increases the PI influx of cells after photodamage treatment.
From: Assembly of Tetraspanin-enriched macrodomains contains membrane damage to facilitate repair
a-c. Tspan4–GFP NRK cells under different cholesterol depletion conditions, including 10% full cholesterol medium (FBS), cholesterol depletion medium (LPDS) and cholesterol depletion medium with 30 μM Pravastatin, were observed by confocal microscopy before and after photodamage. PI influx was used to indicate cell death. Scale bar, 5 µm. Experiments were performed 3 times, and representative images are shown. d. Statistical analysis of the PI intensity of cells under different cholesterol depletion conditions before and after photodamage treatment. Mean±s.e.m., n = 17 cells for FBS, 15 cells for LPDS and 15 cells for LPDS + Prava from 3 independent experiments.
