Extended Data Fig. 4: Tspan4 proteins restrict damage expansion in vitro.
From: Assembly of Tetraspanin-enriched macrodomains contains membrane damage to facilitate repair
a Time-lapse images of Tspan4–GFP GPMVs or GFP-CAAX (as control) GPMVs in the in vitro collapse assay. Green, GFP; red, Rho-PE; yellow, merge. Scale bar, 5 µm. b Diagram of the method used to measure fluorescence enrichment. c Images were collected by confocal microscopy after Tspan4-OE GPMVs, NRK GPMVs and NRK Tspan4-KO GPMVs were used in the in vitro collapse assay. Scale bar, 10 µm; zoom in, 2 µm. d Time-lapse imaging of rupture events in Tspan4–GFP GPMVs after detergent treatment. Yellow arrow, rupture site. Scale bar, 5 µm. Experiments were independently performed 3 times (a and c-d), and representative images are shown.
