Extended Data Fig. 4: HK2 localizes to nucleus of hematopoietic stem/progenitor cells. | Nature Cell Biology

Extended Data Fig. 4: HK2 localizes to nucleus of hematopoietic stem/progenitor cells.

From: The metabolic enzyme hexokinase 2 localizes to the nucleus in AML and normal haematopoietic stem and progenitor cells to maintain stemness

Extended Data Fig. 4

(a) Representative confocal microscopy images of HK2 (green) and Tom20 (red) in FACS sorted CD34+ and CD34- cord blood cells. Scale bar = 10µm. The white (HK2), red (Tom20) and blue (DAPI) curve in the scan profiles represents the fluorescence intensity of HK2, Tom20 and DAPI along the plane. Representative images from 3 biologic samples. (b) Gating strategy for FACs sorted CD34+ and CD34- cord blood cells. (c) Vav promoter flanked cDNA construct of HK2 fused in frame with a nuclear localizing signal. (d) Body weight (n = 42 wild-type control mice, n = 22 Vav-NLS-HK2 mice) and body length (e) (n = 48 wild-type control mice, n = 31 Vav-NLS-HK2 mice) of Vav NLS-HK2 transgenic mice and control littermates. (f) Complete blood count analysis of Vav NLS-HK2 transgenic mice and control littermates (n = 21 wild-type control mice, n = 13 Vav-NLS-HK2 mice). Statistical analyses for experiments (d-e) was performed using an unpaired Student’s t-test. Data represent mean +/- s.e.m.

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