Extended Data Fig. 4: The comparison of oocyte translatomes generated by Ribo-lite, Ribo-tag, Poly-seq and SSP-profiling.

(a) Heat map showing the Spearman correlation between Ribo-lite, Ribo-tag²⁹, Poly-seq²⁷ and SSP-profiling²⁸. NEBD (nuclear envelope breakdown) oocytes²⁸ were released from FGO for 80 min. Results are from two independent experiments. (b) Scatter plots showing the comparison of RNA with translatome in FGOs (or NEBD oocytes for SSP-profiling) in different studies. Red dots represent known dormant mRNAs. The black line represents the linear regression line. Mos and Plat are marked. Results are from two independent experiments. (c) Scatter plots showing the translation efficiency (TE, translatome/mRNA) detected in different studies in FGOs. As mRNA-seq data without selection are unavailable for SSP-profiling, non-polysome mRNA data were used to calculate TE. Right, boxplots showing the TE of dormant RNAs detected in different studies in FGOs. Results are from two independent experiments. N = 17 genes are analyzed for FGO TE. Centre line, median; box, 25th and 75th percentiles; whiskers, 1.5×IQR. (d) Scatter plots showing the comparison of mRNA levels and translatome levels detected by different methods in LPI and MII oocytes. (e) Boxplots showing the TE of dormant RNAs during meiotic resumption. N = 17 genes are analyzed for TE. Centre line, median; box, 25th and 75th percentiles; whiskers, 1.5×IQR. (f) Scatter plots showing the relationship between TE detected by Ribo-lite, Ribo-tag, Poly-seq and SSP-profiling and poly(A) tail length detected by PAIso-seq in FGO⁴⁵. (g) Scatter plots showing the relationship between translatome detected by Ribo-lite, Ribo-tag, Poly-seq and SSP-profiling and protein levels detected by MS in FGOs.