Extended Data Fig. 5: Ribo-lite using 50 HEK293 cells or single oocytes.
(a) Bar plots showing the percentages of sequence reads that are adapters, rRNA reads or clean reads. Representative results are from 19 (for FGOs) and 18 (for MII oocytes) independent experiments. (b) Bar plots showing the number of genes detected by Ribo-lite (FPKM > 1). Representative results are from 19 (for FGOs) and 18 (for MII oocytes) independent experiments. (c) Spearman correlation of RPF signals between low-input (1,000) and 50 HEK293 cells, as well as between low-input FGO and MII oocytes and single oocytes. Representative results are from 19 (for FGOs) and 18 (for MII oocytes) independent experiments. (d) Scatter plots showing the Ribo-lite signal between low-input data and 50 HEK293 cells or single oocytes. The Spearman correlation values are also shown. Representative results are from 19 (for FGOs) and 18 (for MII oocytes) independent experiments. (e) Bar charts showing the RPF metagene distribution around start codon for low-input cells, 50 HEK293 cells, or single oocytes. Position relative to ORFs (0, 1, 2) are shown in different colors. Representative results are from 19 (for FGOs) and 18 (for MII oocytes) independent experiments. (f) Bar plots showing the percentages of footprints that match the reading frames. Representative results are from 19 (for FGOs) and 18 (for MII oocytes) independent experiments. (g) Bar plots showing the percentages of mapped reads with periodicity, as analyzed by RiboCode83. Representative results are from 19 (for FGOs) and 18 (for MII oocytes) independent experiments. (h) Bar plots showing the read coverages at 5′UTR, CDS and 3′UTR regions. Representative results are from 19 (for FGOs) and 18 (for MII oocytes) independent experiments. (i) Box plots showing the RPF levels for known dormant genes using low-input or single-cell oocytes. Centre line, median; box, 25th and 75th percentiles; whiskers, 1.5×IQR. N = 17 genes are analyzed.
