Extended Data Fig. 9: Translation down-regulation during oocyte maturation are mediated by CNOT6L and BTG4.
(a) Line graphs showing the transcription and translation levels of mRNA-destabilized and mRNA-stable genes. GO terms are also shown. Results are from two independent experiments. (b) Bar plots showing the mRNA and translation levels for Cnot6, Cnot6l, Cnot7, Cnot8 and Btg4. Results are from two independent experiments. (c) The UCSC browser views of mRNA-seq signals and Ribo-lite signals of Het oocytes and KO oocytes for Cnot6l and Btg4. Results are from two independent experiments. The knocked out regions are shaded. (d) Venn diagram showing the overlap between RNA targets (up-regulated mRNA DEGs) identified in Cnot6l KO MII oocytes or Btg4 KO MII oocytes and down-regulated mRNA DEGs from FGO to MII stage. Results are from three (for Btg4 samples) and one (for Cnot6l samples) biologically independent experiment. (e) A similar comparative analysis as (d) but for RPF targets or down-regulated RPF DEGs. Results are from three (for Btg4 samples) and one (for Cnot6l samples) biologically independent experiment. (f) Codon adaptation index (cAI) is shown for genes that are regulated by CNOT6L or BTG4, or both, based on Ribo-lite data (results are from three (for Btg4 samples) and one (for Cnot6l samples) biologically independent experiment). P values (two-sided Mann-Whitney-U test) are also shown. Number of genes analyzed here are global (n = 19,952), overlap (n = 55), CNOT6L specific (n = 57) and BTG4 specific (n = 378). Centre line, median; box, 25th and 75th percentiles; whiskers, 1.5×IQR.
