Extended Data Fig. 3: ZBTB43 protein has affinity to methylated and unmethylated genomic DNA and to predicted Z-DNA-forming sequences.
From: Z-DNA is remodelled by ZBTB43 in prospermatogonia to safeguard the germline genome and epigenome
(a-b) Heatmap analysis is shown of MBP-ZBTB43 (FL) binding intensities to genomic DNA, either Dnmt1/Dnmt3a/Dnmt3b triple knockout (TKO) ES cell DNA16 or DNA fully methylated by SssI bacterial CpG methyltransferase (SssI) centered at the TKO DNA peaks (a) and at the SssI peaks (b). Background level binding by MBP is included to the right. (c) Venn diagram shows the relationship between ZBTB43 affinity peaks mapped in SssI and TKO DNA. (d) Venn diagram shows the relationship between ZBTB43 affinity peaks mapped in SssI DNA, TKO DNA, and predicted Z-DNA sites17 or enriched Z-DNA sites in activated B-cells18. (e-f) Heatmaps show the match between ZBTB43 affinity binding peaks and Z-DNA sites. The affinity intensities are centered at predicted Z-DNA sites (e) and enriched Z-DNA sites mapped in activated B-cells (f). (g-h) Venn diagrams displays the relationship between predicted Z-DNA locations and ZBTB43 affinity binding sites in SssI (g) or TKO DNA (h). (i-j) Venn diagrams displays the relationship between in vivo mapped Z-DNA sites in activated B-cells and ZBTB43 affinity binding sites in SssI DNA (i) or TKO DNA (j). Affinity-sequencing results shown represent two biologically independent samples.
