Extended Data Fig. 6: Z-DNA is removed in prospermatogonia between 13.5 and 15.5 dpc.
From: Z-DNA is remodelled by ZBTB43 in prospermatogonia to safeguard the germline genome and epigenome
(a-b) Immunohistochemistry is shown of the Zbtb43+/+ wild-type (a), and Zbtb43-/- mutant (b) fetal testis sections at 13.5, 15.5 and 18.5 dpc using the ZBTB43 (red), and Z-DNA (green) antibodies, counterstained with DAPI. Scale bar: 10 µm. The ZBTB43-Z-DNA double staining was done three times at all stages. (c-e) Quantification of signal intensities of DAPI, Z-DNA and ZBTB43 in Zbtb43+/+ wild-type, and Zbtb43-/- mutant fetal male germ cells shown relative to the corresponding somatic cells at 13.5 dpc (n=10 cells from one out of two independent experiments) and at 15.5 and 18.5 dpc (n=19 cells from one out of two independent experiments). Data are presented as mean values +/- SEM. Statistically significant changes as calculated by multiple 2-sided T-tests are marked with P-value.
