Extended Data Fig. 3: The effects of inhibiting liver differentiation on WT and HHEX KO cells. | Nature Cell Biology

Extended Data Fig. 3: The effects of inhibiting liver differentiation on WT and HHEX KO cells.

From: CRISPR screening uncovers a central requirement for HHEX in pancreatic lineage commitment and plasticity restriction

Extended Data Fig. 3

a,b, Flow cytometry analysis of AFP, PDX1 and CDX2 expression at the PP1 and PP2 stage WT/KO cells using differentiation Condition 1 (a) and Condition 2 (b). c, Quantification of flow cytometry analysis of AFP+, PDX1+ and CDX2 + cells at the PP1 and PP2 stages in both conditions. Each symbol represents one independent experiment (n = 8 independent experiments, except for CDX2 staining, where n = 4 independent experiments) and data are presented as mean ± s.d. Statistical analysis was performed using two-way ANOVA followed by multiple comparisons with Tukey correction. d, Immunostaining images for PDX1, AFP and CDX2 expression at the PP2 stage WT/KO cells using differentiation Condition 2. Images shown represent three independent experiments. Scale bar, 50 μm.

Source data

Back to article page