Extended Data Fig. 7: Multilineage reconstitution from embryonic and adult heart endothelial cell-derived LT-HSCs.

(A) (i) Staining controls using adult C57BL/6 wild-type peripheral blood, spleen, thymus and bone marrow. (ii) Flow cytometry using hematopoietic tissues from recipients 6 months after transplantation with E11.5 endothelium and E11.5 Mesp1^der PSC co-aggregates. (iii) Flow cytometry using hematopoietic tissues from recipients 6 months after transplantation with E13.5 endothelium and E11.5 Mesp1^der PSC co-aggregates. (iv) Flow cytometry using hematopoietic tissues from recipients 6 months after transplantation with adult cardiac endothelium and E11.5 Mesp1^der PSC co-aggregates. (B) (i) Relative donor chimerism and HSC numbers from whole AGM re-aggregates and PSC/endothelial co-aggregates (n = 5). (ii) Table summarizing HSC numbers calculated from serial dilutions/transplantation. (iii) Table summarizing the contribution of donor cells to various blood lineages in recipient mice. The number of HSCs was estimated using a limited dilution assay as described¹⁸ and calculated in Graph Pad Prism v8.4.3 using nonlinear regression. Data were derived from biologically independent samples, animals and experiments. Data represent mean ± S.D.

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