Extended Data Fig. 9: Wnt1der PSCs do not induce EHT.
(A) (i) Schematic diagram showing the experimental strategy used to generate cells for co-aggregation of endothelial cells with Wnt1der PSCs. (ii) CFU-C assays from 96-h co-aggregate cultures (n = 5–7 co-aggregates per culture condition). (iii) Percentage of GFP+ cells in peripheral blood of recipients (n = 5 co-aggregates per culture condition). One co-aggregate per recipient mouse and each point represents an individual recipient. (iv) Flow cytometry analysis of bone marrow, spleen and thymus for GFP+ cells. (B) CFU-C generation was also comparable across different lots of FCS. PSC: PDGFRA + stromal cells; AGM: aorta gonad mesonephros; EC: endothelial cell; HEC: adult heart endothelial cells. ANOVA was used to compare colony counts and donor chimerism. Data were derived from biologically independent samples, animals and experiments. Data represent mean ± S.D.
