Extended Data Fig. 5: Age-associated downregulation of Adar1.
a, Expression of the indicated proteins was determined by immunoblot in the indicated tissues harvested from young (4 months) and aged (24 months) mice. The experiment was repeated two times independently with similar results. b-c, The cortex tissues from brains of young (4 months) and aged (24 months) mice were analysed for expression of the indicated proteins by immunoblot. The intensity of Adar2 (b) or Adar3 (c) immunoblot was quantified by NIH ImageJ software in young and aged mouse cortex tissues and normalized against a loading control β-actin expression. d-g, Expression of Adar1 and Sirt1 was determined by immunoblot in the intestine tissues harvested from young mice (4 months), and aged mice (20 months) treated with either vehicle PBS control or Lys05 (10 mg/kg daily) for 2 weeks (d). The intensity of Adar1 (e) and Sirt1 (f) immunoblot was quantified using NIH ImageJ software in indicated groups by normalizing against a loading control β-actin expression. Correlation between Adar1 and Sirt1 protein expression as determined by a two-sided Pearson correlation analysis (g). n = 5 biologically independent mice per group. h-i, Expression of Adar1 mRNA was analysed by RT-qPCR in ovaries (h) and intestines (i) from young (4 months) and aged mice (20 months) treated with either vehicle PBS control or Lys05. j-m, Expression of p62 was determined by immunoblot in ovaries (j) and intestines (l) from young mice (4 months) and aged mice (20 months) treated with either vehicle PBS control or Lys05. The intensity of p62 immunoblot in ovaries (k) and intestines (m) was quantified using NIH ImageJ software by normalizing against a loading control β-actin expression. n = 5 biologically independent mice per group. Data represent the mean ± s.d. in b, c, e and f, or s.e.m. in h, I, k and m of biologically independent experiments. P values were calculated using a two-tailed Student’s t-test. Source numerical data and unprocessed blots are available in source data.
