Extended Data Fig. 4: ADAR1 regulates SIRT1 expression.
a, Expression of p16INK4a mRNA expression was analysed by RT-qPCR in the indicated IMR90 cells. b, Heatmap visualization of detected (at least 10 read counts and 1 FPKM) and differentially expressed genes (> 2-fold) between IMR90 cells expressing shControl or shADAR1 as determined by the RNA-seq analysis. c, Expression of SIRT1 mRNA was analysed by RT-qPCR in the indicated IMR90 cells. d, Heatmap of HuR-associated mRNAs that are significantly reduced by ADAR1 knockdown in IMR90 cells. List of 5 genes related to autophagy pathway was included in the top and the top 25 of the rest of genes identified were listed. IMR90 cells expressing shHuR were used as a positive control. e-f, Expression of the indicated proteins was determined by immunoblot in the IMR90 cells expressing shControl or the indicated shHuR (e) and relative ADAR1 levels were quantified (f). g, Immunoblot of HuR in IMR90 cells with the indicated treatment immunoprecipitated by an anti-HuR antibody. An isotype matched IgG was used as a negative control. h, Expression of the indicated proteins was determined by immunoblot in the indicated IMR90 cells. Data represent the mean ± s.d. in a and c and ± s.e.m. of three biologically independent experiments. P values were calculated using a two-tailed Student’s t-test. Source numerical data and unprocessed blots are available in source data.
