Extended Data Fig. 7: Lymphocyte subpopulations in control and endometriosis tissues.
a, Density plot showing distribution of lymphocyte cells for each tissue. b, Dot plot representing marker genes for each lymphocyte subpopulation, including four natural killer cell (NK) clusters, innate lymphoid cells (ILCs), effector memory T-cells (TEM), cytotoxic T-lymphocytes (CTL), naïve/central memory T-cells (TN/TCM), T regulatory cells (TReg), CD4- and CD8- tissue resident T cell (CD4-TRM and CD8-TRM, respectively), CD8 mucosal-associated invariant T cells (CD8-MAIT), plasma cells, and B cells. c, Representative IMC images showing the presence and proximity of myeloid cells labelled with CD68 (yellow) with T cells labelled with CD3 (cyan), and TReg labelled with FOXP3 (magenta) in EcO (n = 5); nuclei are marked with DNA intercalation (blue). Scale bar = 100 μm. d, Proportion bar plot of CTLA4 expressing cells from the total TReg subpopulation. e, Proportion box plot of BCL6, SEMA4A, CXCR5 expressing cells from the total B cells within each sample type. For box plots, each dot represents a unique patient (Ctrl n = 3, EuE n = 9, EcP n = 8, EcPA n = 6, EcO n = 4). The box represents the interquartile range with median and minimum/maximum represented by box centerline and whiskers, respectively. Related to Fig. 6.
