Extended Data Fig. 8: Characterization of in vivo epithelial and in vitro endometrial epithelial organoid (EEO) cells. | Nature Cell Biology

Extended Data Fig. 8: Characterization of in vivo epithelial and in vitro endometrial epithelial organoid (EEO) cells.

From: Single-cell analysis of endometriosis reveals a coordinated transcriptional programme driving immunotolerance and angiogenesis across eutopic and ectopic tissues

Extended Data Fig. 8

a, Proportions of epithelial subpopulations per sample type. b, Representative IMC images of MUC5B+ epithelial cells in eutopic endometrium (Ctrl: C07, EuE: E12, E06) from multiple tissues. Epithelial cells are marked with PanCK, EpCAM, E-cadherin (green); MUC5B (magenta); nuclei (white). Scale bar = 100 μm. c, Proportion box plot of SAA1 expressing cells from the total MUC5B+ cells within each sample type. Each dot represents a unique patient (Ctrl n = 3, EuE n = 9, EcP n = 8, EcPA n = 6, EcO n = 4). The box represents the interquartile range with median and minimum/maximum represented by box centerline and whiskers, respectively. d, Sequencing metrics from EEO scRNA-seq; UMIs and unique genes counts are shown for Control (C) and endometriosis (E) patients and across tissue type. Undetermined (UD) group represents single cells which could not be assigned due to the lack of multiplexing hashtag but otherwise passed QC. e, Density plot showing distribution of EEO cells derived from Ctrl, EuE, EcP, and EcPA (UD cells were not included). f, UMAP showing the co-expression of MUC5B, SAA1, TFF3, and RUNX3 in the MUC5B+ population comprising in vivo epithelial cells and EEO. Related to Fig. 7.

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