Extended Data Fig. 2: Proportion of major cell types in each replicate and IMC panel for spatial profiling of Ctrl and endometriosis tissues.

a, Major cell types were determined based on UMAP. The mean distribution for all 5 major cell populations is represented for each tissue type Ctrl, EuE, EcP, EcPA, and EcO (left of the line). Each pie chart represents major cell type proportions for each replicate (right of the line). b, Each antibody was selected according to the cell types identified by the scRNA-seq data analysis. Representative images show single channels for each metal-conjugated antibody in a EuE biopsy. A total of 26 antibodies was used to identify cellular heterogeneity within stromal, endothelial, epithelial, lymphocyte, and myeloid major cell types. Additional antibodies (in ‘Others’) were used to identify cell proliferation (Ki67), active metabolism (pS6), extracellular matrix (Collagen1), and nuclei (DNA). A complete list of cell subpopulations identified through this panel of markers is listed on Supplementary Table 8b. Related to Fig. 1.