Extended Data Fig. 4: Cadherin heterogeneity within the same cell population in ETX embryos. | Nature Cell Biology

Extended Data Fig. 4: Cadherin heterogeneity within the same cell population in ETX embryos.

From: Stem cell-derived synthetic embryos self-assemble by exploiting cadherin codes and cortical tension

Extended Data Fig. 4

(a) Pie charts show different mis-sorted ETX embryos under the indicated conditions. n = 4186 (control), n = 2940 (Cdh1-KD ES), n = 2471 (Cdh3-KD ES), n = 2407 (Cdh1-KD TS), n = 2151 (Cdh3-KD TS) structures were collected from 3 independent experiments for quantification. (b) Immuno-staining of ES (upper) and TS (lower) cells to reveal E-cadherin (green) and P-cadherin (red), respectively. Nuclei (purple) are stained by DAPI. Scale bars represent 100 μm. Zoomed images are of regions indicated by dashed lines. Experiments were repeated 5 times. (c) Flow cytometric analysis of E-cadherin in wild-type ES cells, E-cadherin knockdown ES cells and ES cells over-expressing E-cadherin (upper). Flow-cytometric analysis of P-cadherin in wild-type TS cells, P-cadherin knockdown TS cells and TS cells over-expressing P-cadherin (lower). CV (coefficient of variation) values shown against peak values in plots. (d) Top: FACS profiles for E-cadherin in E-cadherin KD, WT and E-cadherin OE ES cells, Bottom: FACS profiles for P-cadherin in P-cadherin KD, WT and P-cadherin OE TS cells.

Source data

Back to article page