Extended Data Fig. 9: HASTER perturbations in β cells.
From: The HASTER lncRNA promoter is a cis-acting transcriptional stabilizer of HNF1A
a,b, HASTER and HNF1A RNA in EndoC-βH3 cells with clonal homozygous deletion of (a) HASTER P1 promoter (HASTERΔP1/ΔP1) or (b) HASTER P1 and P2 promoters (HASTERΔP/ΔP). Deletion #1 and #2 were generated with independent pairs of sgRNAs, HASTER+/+ clones were transfected with sgRNAs targeting the AAVS1 locus. n = 4 clones per deletion. c, Two sets of LNA oligonucleotides (GapmeRs) were used to elicit HASTER degradation in EndoC-βH3 cells, without significant changes in HNF1A or HNF4A mRNA. n = 3 nucleofections. a-c, Expression normalized by TBP. Mean ± s.d., two-tailed Student’s t-test. d, Haster activation by CRISPR-SAM in MIN6 mouse β cells had no effect on Hnf1a expression. n = 3 lentiviral transductions. Expression normalized by Tbp. Mean ± s.d., two-tailed Student’s t-test relative to the control #1 sgRNA.
