Extended Data Fig. 1: Characterization of hiPSC-derived branching structures.
a, Phase-contrast representative images of aggregates were differentiated in chemically defined medium, treated with SB431542 on Day 2 and monitored during Days 0–10. Representative of 18 independent experiments. Scale bars, 200 μm. b, RT–PCR revealed the gene expression levels of salivary gland cell lineage markers in organoids on Day 60 and undifferentiated human iPSCs (201B7). c, Immunofluorescence images of the salivary gland organoids on Day 60. The epithelial marker pan-CK, ductal marker CK18, basal cell marker CK5, myoepithelial cell marker α-SMA, and salivary gland transcription factor FOXC1 were detected. Scale bars, 200 μm.
