Extended Data Fig. 3: Orthotopic transplantation of human salivary gland organoids.
a, Real-time RT–PCR analysis of salivary gland marker genes. Data are normalized to ribosomal protein L27 and presented as mean values + /− SD (n = 3 biological replicates). Statistically significant changes as calculated by one-way ANOVA followed by Tukey’s post hoc test. b, Immunofluorescence images of the transplantation site. Mouse CD31+ vessels (left) and TUBB3+ nerve fibres (right) were observed in the mesenchyme around the Pan-CK+ parenchyma derived from hiSGs. Scale bars, 50 μm. Representative of three independent experiments.
