Extended Data Fig. 6: Migrasome triggers VEGFR2 and AKT phosphorylation.
From: Monocytes deposit migrasomes to promote embryonic angiogenesis
(a) Cells were treated with migrasome or VEGF165 for 24 hours and the Lysates of cell were analysed by western blot using indicated antibody. 3 repeats were shown (repeat 1 is figure 5q). (b) The relative fold change of indicated signal in the control, VEGF165 and migrasome treated cells was quantified. Data are presented as mean ± SEM; n = 3 from three independent experiments; P values were calculated using a one-way ANOVA unpaired multiple comparisons. VEGFR2 protein level: (N.S: no significance; P=0.9981, Control/control vs. VEGF/control; P=0.9725, Control/control vs. Mig/control). p-VEGFR2 protein level: (P=0.0325, Control/control vs. VEGF/control; P=0.0037, Control/control vs. Mig/control). AKT protein level: (N.S: no significance; P=0.6451, Control/control vs. VEGF/control; P=0.2196, Control/control vs. Mig/control). p-AKT protein level: (P=0.0263, Control/control vs. VEGF/control; P=0.0263, Control/control vs. Mig/control). Source numerical data and unprocessed blots are available in source data.
