Extended Data Fig. 8: TSPAN4 KO impairs migrasome formation.
From: Monocytes deposit migrasomes to promote embryonic angiogenesis
(a) Diagram showing the strategy for knocking out TSPAN4 and knocking in mCherry in chick embryos. The mCherry coding sequence was inserted into TSPAN4 at the position targeted by the sgRNA. Thus, an mCherry-positive signal indicates that native TSPAN4 gene expression was silenced simultaneously. (b) Time-lapse imaging of migration and migrasome formation by WGAhigh cells isolated from WT or TSPAN4-KO CAM8d. Cells were stained by WGA and observed by spinning disk microscopy. Scale bar, 5 µm. (c) CAMs from Fig. 6k and 6l were stained for WGA and observed by spinning disk microscopy. Scale bar, 30 µm. (d) CAMs from Fig. 6k and 6l were quantified for the number of WGAhigh cells. Data are presented as mean ± SEM; n = 15 cells from three independent experiments; P values were calculated using a two-tailed, unpaired t-test, P < 0.0001. Source numerical data are available in source data.
