Extended Data Fig. 8: Additional immunofluorescence images showing that the AJ-mediated membrane compartmentalization modulates neural progenitor cell (NPC) differentiation.
From: Adherens junctions organize size-selective proteolytic hotspots critical for Notch signalling
(a) Immunostaining of the subventricular zone (SVZ) in the lateral ventricle (LV) of the E13.5 DAPT-treated mouse brain. Notch was colocalized at NAJ, visualized by immunostaining with anti-N-cadherin and anti-Notch1 antibodies. (i) Representative lower magnification image. The indicated area (a white box) is magnified and rotated 90° clockwise in the lower panel. Scale bar, 100 µm. (ii) Magnified view of the region indicated with a white dashed box in the (i) lower panel. Scale bar, 2.5 µm. (iii) Line profiles of N-cadherin and Notch distributions. (b) Additional confocal images of coronal sections of developing mouse brain retrovirally infected with dominant negative form of E-cadherin vector (DN-Ecad-EGFP). Transduced cells differentiated into post-mitotic neurons can be identified as EGFP + /Tuj1 + , while those remained as NPCs with plasmid transfection are only EGFP + . (left) Low-magnification images. Insets show the magnified image of a representative single cell immunostained for myc-tag. (right) Magnified view of the boxed region. Scale bar, 50 µm. (c) Ratio of GFP/Tuj1-double positive cells to total Tuj1-positive post-mitotic neurons in these two conditions. Data are the mean ± s.e.m. of n = 3 (vector control) and 5 (DN-Ecad-EGFP) biologically independent animals. Two-tailed unpaired Student’s t test.
