Extended Data Fig. 3: Characterization of multivalent interactions among EB1 regions by NMR.
From: Phase separation of EB1 guides microtubule plus-end dynamics
(a) Combined chemical shifts perturbation (CSP) of IDR in the presence of EBH (IDR vs IDR + EBH). The dash lines indicated the level of standard deviation values. Proteins boxed in red were labeled by 15N. Gray bars indicated overlapping or unassigned peaks, while the asterisks labeled the proline residues which had no signal in NMR assay. (b) Combined chemical shifts perturbation of IDR + EBH in the presence of CTT (IDR + EBH vs IDR + EBH + CTT. *, proline residues. (c) Peak intensity change of IDR + EBH + CTT (15N labeling) upon CH domain titration. The error bars were calculated based on every peak’s signal-to-noise ratios as described in Methods. *, proline residues. (d) Representative spectra of indicated regions were overlaid to show the peak shifts. The spectra of IDR, IDR + EBH, and IDR + EBH + CTT were shown in red, blue, and green, respectively. The peak shifts of residues showed the interaction between indicated regions. (e) Summary of the interactions among different EB1 regions.
