Extended Data Fig. 6: Extended Data Fig. 6, Laurdan studies.
From: Caveolin-1 dolines form a distinct and rapid caveolae-independent mechanoadaptation system
(A) A representative of PTRFKO + PTRF MEFs before, during (10% for 60 min), and after stretch for channels centered at 440 nm (Ch1) and 490 nm (Ch2), GP of the whole cell, GP of the border of the cell at a certain z-plane, and the corresponding histogram with Gaussian fitting. (B) Variance of the change in GP with the angle for different time points during the experiment for PTRFKO + PTRF MEFs (n = 5 independent experiments, each of them with one cell analyzed over the indicated time points), D: during stretching, B: before stretching, D10, D20, D30, D40, D50: during stretching for 10, 20, 30, 40 or 50 minutes, A: after stretching, A20: after 20 min post stretching. Data are presented as mean values ± s.e.m. Statistical comparisons were by paired two-tailed Student’s t-test, with significance assigned at *p < 0.05, **p < 0.01, ***p < 0.001 and not significant at p > 0.05. (C) Schematic of the multi-photon set-up. (D) Schematic of the stretching device. (E) Plot of the intensity of the fluorescence as function of the input power showing three-photon behavior. Source numerical data are available in source data.
