Extended Data Fig. 6: Murine OASL1 is required for MCMV infection-induced necroptosis to restrict viral dissemination in vivo.
a, Measurement of footpad swelling caused by subcutaneous footpad injection of Ripk3+/+ or Ripk3-/- mice with 106 PFU (left) MCMV-WT (n = 4 per genotype) or (right) MCMV-M45mutRHIM (Ripk3+/+: n = 5, except n = 3 for 6 d.p.i.; Ripk3-/-: n = 5 for 0-4, 10 d.p.i., n = 3 for 6,8,11 d.p.i.). Data are represented as the percent increase in thickness relative to the pre-injection measurement and plotted for mean values at the indicated times. b,c, Body weight changes of a Ripk3+/+ and Ripk3-/- mice infected with MCMV-WT (n = 4 per genotype) and MCMV-M45mutRHIM infection (n = 5 per genotype) and Fig. 6a,b Oasl1+/+ and Oasl1-/- littermate mice infected with MCMV-WT (n = 8 for Oasl1+/+ and n = 6 for Oasl1-/-) or MCMV-M45mutRHIM (n = 8 mice per genotype). d,e, Viral titers in salivary glands (SG) from Oasl1+/+ and Oasl1-/- littermate mice infected with (d) MCMV-WT (n = 3 mice per genotype) or (e) MCMV-M45mutRHIM (n = 2 for Oasl1+/+ and n = 3 for Oasl1-/-) at the indicated days post-footpad subcutaneous injection. f, IL-1α levels in the sera of Oasl1+/+ and Oasl1-/- mice infected with MCMV-WT or MCMV-M45mutRHIM at the indicated days were determined by ELISA (n = 3 mice per genotype). d-f, Each symbol represents one mouse, horizontal lines represent the mean value. g,h, Quantification of Fig. 6e cell infiltration (n = 8 per genotype for PBS, n = 17 per genotype for MCMV-WT) and footpad epidermal thickness (n = 31 per genotype for PBS, n = 41 per genotype for MCMV-M45mutRHIM) in mice infected with MCMV-WT or MCMV-M45mutRHIM at 2 or 6 d.p.i., respectively. All data are pooled from three independent experiments. d-h Data are presented as mean ± SEM. Statistical analyses were performed using a two-tailed unpaired t-test (d-f) or two-way ANOVA (a,g,h).
