Extended Data Fig. 6: Extended Data Related to Fig. 6.
From: Quantifying propagation of DNA methylation and hydroxymethylation with iDEMS
a) Schematic showing histone PTM localization at the replication fork. b) Hydroxymethylation levels in H3K9me3-, H3K36me3-, H4K20me2-, H4K20me0-, and H4K5ac-associated DNA, compared to gDNA. c) Methylation levels in Fig. 5c (ChIP-iDEMS EdU+ ssDNA) shown as a percentage of methylation measured in the corresponding ChIP-MS dsDNA. EdU+ ssDNA shown as a percentage of gDNA. In b, c), data are presented as mean values +/− SD from three biological replicates. P-values calculated using one-way ANOVA, adjusting for multiple comparisons: *, p < 0.05; **, p < 0.01; ***; p < 0.001 ****, p < 0.0001. Exact adjusted p-values for b): total gDNA v H3K9me3 DNA: p = 0.0273; total gDNA v H3K36me3 DNA: p = 0.0112; total gDNA v H4K20me2 DNA: p = 0.8763; total gDNA v H4K20me0 DNA: p = 0.9986; total gDNA v H4K5ac DNA: p = 0.8763. Numerical source data are provided.
