Fig. 4: DNA methylation levels are not restored by mitosis.
From: Quantifying propagation of DNA methylation and hydroxymethylation with iDEMS
a, Sorting strategy to isolate pure populations of mESCs in G1 and G2/M. Numbers represent the percentage of cells in each gate. PI, propidium iodide. b, Percentage of CpG methylation genome wide in G2/M versus G1 cells. Red dots: G1 > G2/M DMRs; blue dots: G1 < G2/M DMRs. c, Stacked bar chart showing proportion of replication domains overlapping quantifiable 100 CpG windows globally (left), in G1 > G2/M DMRs (centre) and in G1 < G2/M DMRs (right). d, CpG methylation levels in early (left) and late (right) replication domains in sorted G2/M and G1 populations. Black line: median; boxes: 25th–75th percentiles; dashed lines: 1.5× interquartile range. P-values are indicated in each plot. EM-seq data in b–d calculated using 100-CpG windows quantified in all datasets. DMRs identified using logistic regression, P < 0.05. P values calculated using two-sided Mann–Whitney U test. Replication timing data from ref. 45. Numerical source data are provided.
