Extended Data Fig. 3: Zfp462 expression analysis and impact of Zfp462 deletion on ESC morphology and gene expression. | Nature Cell Biology

Extended Data Fig. 3: Zfp462 expression analysis and impact of Zfp462 deletion on ESC morphology and gene expression.

From: ZFP462 safeguards neural lineage specification by targeting G9A/GLP-mediated heterochromatin to silence enhancers

Extended Data Fig. 3

a) UMAP plots visualize lineage assignments of cells in mouse embryonic developmental stages (embryonic day (E) E4.5 cells, E5.5 cells and E6.5-7 cells) as previously described (Wolf Reik et.al) (top). UMAP plots of Zfp462 RNA expression at corresponding developmental stages (bottom). b) Sanger sequence chromatograms of heterozygous Zfp462 mutants. Heterozygous non-sense mutations are highlighted in grey. c) Alkaline phosphatase staining of Zfp462 +/Y1195* and Zfp462 −/− cl.2 ESCs (scale bar = 100 μm). d) Correlation plot shows principal component analysis (PCA) of replicate RNA-seq experiments from WT, two Zfp462 +/− and two Zfp462 −/− ESC lines. e) Volcano plot shows differential gene expression of Zfp462 −/− cl.2 ESCs compared to WT ESCs. (n = three replicates). f) Bar plots show gene ontology (GO) terms enriched in the four clusters of heatmap in Fig. 3e.

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