Extended Data Fig. 4: Lineage-specifying genes are deregulated during neuronal differentiation.
a) Alkaline phosphatase staining of WT and Zfp462 mutant ESCs cultured in 2i/LIF/Serum medium (scale bar = 180 μm). b) Representative bright field images show Zfp462 +/Y1195* and Zfp462 −/− cl.2 at corresponding stages of neural differentiation. Scale bar = 100μm. c) Western blot analysis shows levels of ZFP462, G9A and H3K9me2 in WT and Zfp462 −/− ESCs during neural differentiation. LMNB1 is used as loading control. d) Line plot shows RT-qPCR analysis of Zfp462 RNA expression during neural differentiation (n = two replicates). Expression level is shown relative to ESCs (2i/S/L). e) Heatmaps show differential expression of selected marker genes specific for endodermal (EN), mesodermal (ME) and neural lineages (EC) in heterozygous and homozygous Zfp462 mutant cells during neural differentiation.
