Extended Data Fig. 3: miR-139-5p downregulates LPAR4 expression in pancreatic cancer cells.
a, Graphs showing the log2-FC of miR-139-5p expression level in cells with or without LPAR4 expression manipulation. b, Graph shows the log2-FC of mRNA level of LPARs in Colo-357 cells treated with anti-miR-139-5p, normalized to cells treated with scrambled control miRNA. Data were presented as mean ± s.d. for n = 6 independent experiments for LPAR4 and n = 5 for other LPARs. c, Construct map for LPAR4-3’UTR luciferase reporter vector or control vector. d, Immunoblots showing representative of three independent experiments for LPAR4 protein level in Colo-357-sh-CTRL or sh-R4.1 cells treated with scrambled control miRNA vs. anti-miR-139-5p. e, Graphs showing the log2-FC of mRNA level of LPAR4 and various predicated miR-139-5p targets in Colo-357 or 34E cells treated with miR-139-5p mimic, normalized to cells treated with scrambled control miRNA. Data were presented as mean ± s.d. for n = 3 independent experiments (a, b, and e). Statistical analyses were performed using two tailed unpaired one sample t-test (a, b, and e). Source numerical data are available in source data.
