Extended Data Fig. 5: LPAR4 induces the expression of FN1 isoforms containing EDA and EDB domains.
a, TNMplot showing FN1 gene expression is significantly higher in pancreatic adenocarcinoma (PAAD) than in normal pancreas (P < 0.0001). The unpaired two-tailed t test was used for statistical analysis. Bars represent median values for each group. b, Graphs showing the relative mRNA level of total FN1, FN1 containing EDA domain (FN1-EDA), and FN1 containing EDB (FN1-EDB) in +EV vs. +R4 cells as indicated. All mRNA expression was normalized to +EV cells. Expression of FN1-EDA or FN1-EDB is quantitated independently by using two different sets of primers. c, Graphs showing the relative LPAR4 mRNA level in cells stably transfected with scrambled shRNA (sh-CTRL) or two different LPAR4 shRNAs (sh-R4.1 and sh-R4.2), treated with hypoxia and no serum for 72 h. All were normalized to LPAR4 mRNA level in normoxia and no serum condition. Data were presented as mean ± s.d. for n = 3 biological experiments (b and c). P-value was calculated using two tailed unpaired one sample t-test. Source numerical data are available in source data.
