Extended Data Fig. 4: Depletion of Menin or LEDGF enhances basal and IFN-γ-induced bivalent MHC-I gene expression.
From: Targeting Menin disrupts the KMT2A/B and polycomb balance to paradoxically activate bivalent genes
a,b, Cell surface MHC-I in K-562 Cas9 cells transduced with control, MEN1 or PSIP1 sgRNA. b, Bars show mean percentage of MHC-I expression from three experiments, indicated by points. Unpaired two-tailed Student’s t-test, significant changes are indicated, P = 0.0356. c, qRT-PCR for MHC-I genes in K-562 Cas9 cells transduced with control or MEN1 sgRNA. Bars indicate mean ± s.d. of technical triplicates from a representative experiment. d, Immunoblot of K-562 Cas9, MEN1-KO and PSIP1-KO cells ± 10 ng ml−1 IFN-γ for 40 h. e, Cell surface MHC-I in K-562 Cas9 cells transduced with control or the indicated sgRNA targeting MEN1. f,g, Immunoblots of K-562 Cas9 cells transduced with control sgRNA or sgRNA targeting MEN1 (f), MEN1-KO cells ± MEN1 cDNA (g). h,i, JunD is not required for enhanced MHC-I expression following MEN1 KO. K-562 Cas9 and MEN1-KO cells transduced with control or JunD sgRNA and analysed by flow cytometry following treatment with 10 ng ml−1 IFN-γ for 48 h (h) and immunoblot (i). h, Representative plots from three experiments (Supplementary Fig. 3).
