Extended Data Fig. 9: KMT2A/B is required for basal MHC-I expression.
From: Targeting Menin disrupts the KMT2A/B and polycomb balance to paradoxically activate bivalent genes
a, Cell surface MHC-I in K-562 Cas9 cells transduced with KMT2A or KMT2B sgRNA compared with control sgRNA and treated with 10 ng ml−1 IFN-γ for 48 h. Bars show mean percentage of MHC-I expression from three experiments, indicated by points. Unpaired two-tailed Student’s t-test compared with control sgRNA. Significant changes are indicated; P < 0.0001. b,c, Immunoblots in K-562 Cas9 and KMT2B-KO cells (b), and KMT2A-KO ± KMT2B-KO cells (c). d, Cell surface MHC-I in K-562 KMT2B + PCGF1-KO cells transduced with the indicated sgRNA and treated for 5 d with DMSO, 1 µM VTP50469 or 3 µM EPZ-011989. Representative plot from three experiments (Supplementary Fig. 3). e, Genomic snapshots of H3K4me3 CUT&Tag in K-562 Cas9 and KMT2A/B-KO cells treated ± EPZ-011989. The EZH2i-treated (no IFN-γ) track is also shown in Fig. 8g. f, Immunoblots in K-562 Cas9, MEN1-KO and KMT2A-KO cells. g–i, Genomic snapshots of K-562 Cas9 and MEN1-KO cells (g,h) H3K4me3 ChIP–seq and KMT2A CUT&RUN (i). The H3K4me3 tracks are also shown in Extended Data Fig. 7h.
