Extended Data Fig. 10: KMT2A/B is dispensable for MHC enhanceosome-driven activation.
From: Targeting Menin disrupts the KMT2A/B and polycomb balance to paradoxically activate bivalent genes
a, Schematic overview of cis-regulatory elements in the MHC-I promoter. NLRC5 forms an enhanceosome with the RFX (regulatory factor X) complex, made up of RFX5, RFXANK and RFAXP (RFX-associated ankyrin-containing protein); CREB (cAMP-responsive-element-binding); and NFY (nuclear transcription factor Y), which bind the SXY-molecule to activate transcription of MHC-I. b, Immunoblot of K-562 Cas9 cells transduced with control and RFX5 sgRNA. c, IFN-γ time course in K-562 Cas9 and the indicated KO cells treated with 3 µM EPZ-011989 and 25 ng ml−1 IFN-γ for the indicated time periods. d, Immunoblot of K-562 Cas9 and KMT2A/B-KO cells transduced with control, SETD1A and/or SETD1B sgRNA.
